| 引用本文： |
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张洁,苗伟,刘畅,等.丁酸盐激活CaMKK促进紧密连接重组装的研究[J].同济大学学报（医学版）,2015,36(5):8-12. [点击复制]
- ZHANG Jie,MIAO Wei,LIU Chang,et al.Butyrate facilitates tight junction reassembly via activation of Ca2+/calmodulin-dependent protein kinase[J].同济大学学报（医学版）,2015,36(5):8-12. [点击复制]
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| 摘要: |
| 目的 探讨丁酸盐激活AMPK从而调节紧密连接的重组装的途径。方法 使用丁酸盐、CaMKK特异抑制剂(STO-609)处理上皮屏障模型Caco-2细胞,在转钙过程中检测电阻(TER)值、细胞内ATP水平以及CaMKK和AMPK的磷酸化状况,从而判断丁酸盐促进紧密连接重组装的上游信号通路。结果 丁酸盐处理Caco-2细胞后,其TER值显著增高(P＜0.05),CaMKK和AMPK均被磷酸化激活,而上述作用可被STO-609部分减弱。结论丁酸盐可通过钙离子途径磷酸化激活CaMKK,继而活化AMPK促进紧密连接的重组。 |
| 关键词: 丁酸盐 Caco-2细胞 紧密连接 |
| DOI：10.16118/j.1008-0392.2015.05.002 |
| 投稿时间：2015-04-21 |
| 基金项目:国家自然科学基金(31170791) |
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| Butyrate facilitates tight junction reassembly via activation of Ca2+/calmodulin-dependent protein kinase |
| ZHANG Jie,MIAO Wei,LIU Chang,KANG Zhi-qian,LI Li,PENG Lu-ying |
| (Dept. of Pathology and Pathophysiology, Medical College, Tongji University, Shanghai 200092, China) |
| Abstract: |
| ObjectiveTo investigate the effect of butyrate on tight junction reassembly and related pathway. Methods The Caco-2 cell monolayer model was treated with butyrate and CaMKK inhibitor STO-609; then the transepithelial electrical resistance(TER), cellular ATP level, phosphorylated AMPK and CaMKK levels were measured. ResultsWith the treatment of butyrate, the TER of Caco-2 cells significantly increased(P＜0.05), phosphorylation of CaMKK and AMPK was increased; and those effects were partly abolished by STO-609. Conclusion Butyrate may regulate the activation of AMPK through phosphorylation of CaMKK, thus facilitate the reassembly of tight junction. |
| Key words: butyrate Caco-2 cell tight junction |
