| 引用本文： |
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朱军,赵希敏.肉苁蓉总苷预处理对缺血再灌注肾损伤保护作用的实验研究[J].同济大学学报（医学版）,2015,36(2):37-40. [点击复制]
- ZHU Jun,ZHAO Xi-min.Protective effect of cistanche total glycosides pretreatment againstrenal ischemia reperfusion injury in rats[J].同济大学学报（医学版）,2015,36(2):37-40. [点击复制]
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| 摘要: |
| 目的 建立大鼠失血性休克复苏模型,观察肉苁蓉总苷(cistanche total glycosides, CTG)对缺血再灌注肾损伤的保护作用。方法 将SD大鼠随机分为3组: 假手术组、实验组(休克复苏组)、CTG处理组,每组各10只。实验组和CTG处理组建立肾缺血-再灌注模型,治疗组大鼠给予CTG 400mg/kg腹腔注射,实验组和假手术组注射生理盐水。分别检测各组血肌酐含量、尿素氮含量、胱抑素C以测定肾功能,流式细胞分析技术检测肾脏细胞凋亡率；酶联免疫法(ELISA)检测大鼠肾组织X-染色体连锁凋亡抑制蛋白(X-linked inhibitor of apoptosis protein, XIAP)表达。结果 CTG组与实验组比较,血Cr、BUN和胱抑素C均明显降低(P<0.01),肾脏细胞凋亡减少(P<0.05),CTG可显著提高XIAP的表达(P<0.05)。结论 肉苁蓉总苷对大鼠缺血再灌注肾损伤具有保护作用,可能与促进XIAP的表达有关。 |
| 关键词: 肾 缺血再灌注损伤 肉苁蓉总苷 染色体连锁凋亡抑制蛋白 细胞凋亡 大鼠 |
| DOI：10.16118/j.1008-0392.2015.02.009 |
| 投稿时间：2014-11-20 |
| 基金项目:上海市科学技术委员会科研计划项目(034119942) |
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| Protective effect of cistanche total glycosides pretreatment againstrenal ischemia reperfusion injury in rats |
| ZHU Jun,ZHAO Xi-min |
| (Dept. of Surgery, Baoshan District Hospital of Integrated TCM and Western Medicine, Shanghai 201900, China) |
| Abstract: |
| Objective To investigate the effect of cistanche total glycosides (CTG) on renal ischemia reperfusion injury in rats. Methods Thirty Sprague-Dawley rats were randomly divided into 3 groups: sham operation group, model group and CTG group with 10 in each. Renal ischemic and reperfusion injury was induced by hemorrhagic shock and resuscitation in rats of model and CTG groups. Intraperitoneal injection of 400mg/kg CTG was given to rats in CTG group, and injection of 3ml normal saline was given to rats in model and sham operation groups. The serum creatinine (Cr), blood urea nitrogen (BUN), and cystatin C levels were measured, apoptosis of renal cells was detected with flow cytometry; the expression of X-linked inhibitor of apoptosis protein(XIAP)in renal tissue was determined with ELISA method. Results Compared to model group, blood Cr, BUN and cystatin C in CTG group were significantly decreased (P<0.01), renal cell apoptosis was attenuated (P<0.01), and the expression of XIAP was increased (P<0.05). Conclusion CTG can protect renal tissue against ischemia reperfusion injury, which may be related to up-regulation of the expression of XIAP. |
| Key words: kidney ischmia reperfusion injury cistanche total glycosides X-linked inhibitor of apoptosis protein apoptosis rats |
