| 引用本文： |
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闻静,张敬莹,仓彦,等.高渗盐水预处理在大鼠缺血再灌注心肌损伤中的保护作用[J].同济大学学报（医学版）,2015,36(2):20-23. [点击复制]
- WEN Jing,ZHANG Jing-ying,CANG Yan,et al.Protective effect of hypertonic saline preconditioning on the rat myocardial ischemia and reperfusion injury[J].同济大学学报（医学版）,2015,36(2):20-23. [点击复制]
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| 摘要: |
| 目的研究高渗盐水(hypertonic saline, HTS)预处理对大鼠缺血再灌注心肌的保护作用及相关机制。方法健康雄性SD大鼠随机分为4组: 假手术组(SO组)、高渗盐水+假手术组(HTS-SO组)、缺血再灌注组(I/R组)、高渗盐水+缺血再灌注组(HTS-I/R),每组10只。大鼠缺血再灌注前经腹腔注射生理盐水或高渗盐水(4ml/kg,7.5%)进行预处理；建立大鼠心肌缺血再灌注(I/R)模型。检测各组大鼠的心肌梗死范围、心肌酶(CK、LDH)、炎症介质(TNF-α、IL-6)、氧化应激指数(MDA、SOD)等的变化。结果与对照组比较,高渗盐水显著性抑制大鼠梗死心肌的范围(34.8%±2.0% vs. 51.5%±1.8%)、心肌酶的释放(LDH: 1120.5±90.5 vs.1950.5±95.0；CK:1658.8±50.0 vs. 2510.5±60.8)、炎症介质的释放(TNF-α: 25.90±0.20 vs. 40.20±0.20；IL-6: 35.7±0.20 vs. 58.20±0.10)并有效调节氧化应激指数(MDA: 7.00±0.50 vs. 12.00±0.50；SOD: 165.50±5.0 vs. 96.50±6.85)(P均＜ 0.05)。结论高渗盐水预处理对大鼠心肌缺血再灌注损伤具有保护作用,其保护机制可能与其抑制炎症因子的释放和调节氧化应激有关。 |
| 关键词: 高渗盐水 缺血再灌注损伤 炎症 氧化应激 大鼠 |
| DOI：10.16118/j.1008-0392.2015.02.005 |
| 投稿时间：2014-09-18 |
| 基金项目:国家自然科学基金(30800466) |
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| Protective effect of hypertonic saline preconditioning on the rat myocardial ischemia and reperfusion injury |
| WEN Jing,ZHANG Jing-ying,CANG Yan,LI Xian-kai,ZHAO Dong-dong,ZHANG Ji |
| (Dept. of Cardiology, Tenth People's Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| ObjectiveTo investigate the effect of hypertonic saline(HTS) preconditioning against myocardial ischemia-reperfusion injury(I/R) in rats. MethodsForty healthy male SD rats were randomly divided into four groups: sham operation group(SO); hypertonic saline+sham operation group(HTS-SO); ischemia and reperfusion group(I/R) and hypertonic saline+ischemia and reperfusion group(HTS-I/R). Myocardial I/R was induced by ligation of left anterior descending(LAD) artery and reperfusion for 4h. Hypertonic saline(4ml/kg, 7.5%) was injected in rats of HTS-SO and HTS-I/R groups before ischemia, respectively. The myocardial I/R injury and oxidative stress were assessed. ResultsCompared to I/R groups, the myocardial infarct size in HYS-I/R group was attenuated(34.8%±2.0% vs. 51.5%±1.8%, P＜0.05), myocardial enzymes were reduced(LDH: 1120.5±90.5 vs 1950.5±95.0; CK: 1658.8±50.0 vs. 2510.5±60.8, P＜0.05), proinammation cytokines were decreased(TNF-α: 25.90±0.20 vs 40.20±0.20; IL-6: 35.7±0.20 vs 58.20±0.10, P＜0.05), and oxidative stress was also reduced(MDA: 7.00±0.50 vs 12.00±0.50; SOD: 165.50±5.0 vs 96.50±6.85, P＜0.05). ConclusionHypertonic saline has a protective effect against myocardial I/R injury in rats, which may be related to anti-inflammation and reducing reactive oxygen species(ROS). |
| Key words: hypertonic saline ischemia-reperfusion injury inflammation oxidative stress rats |
