| 引用本文： |
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高艳,程晨,李静,等.LncRNA AK051397在BMP-2诱导的C2C12成骨分化中的作用[J].同济大学学报（医学版）,2014,35(4):23-28. [点击复制]
- GAO Yan,CHENG Chen,LI Jing,et al.Involvement of LncRNA AK051397 in BMP-2 induced-osteogenic differentiation of myoblast C2C12 cells[J].同济大学学报（医学版）,2014,35(4):23-28. [点击复制]
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| 摘要: |
| 目的研究骨形态发生蛋白2（bone morphogenetic protein-2,BMP-2）诱导后表达上调的LncRNA AK051397在C2C12成骨分化过程中的作用。方法BMP-2诱导C2C12成骨分化过程,实时定量PCR技术与碱性磷酸酶（ALP）染色检测成骨相关指标。对C2C12细胞在BMP-2诱导下,成骨分化过程中的LncRNAs表达变化进行芯片分析（ArrayStar LncRNA Array）,筛选出表达明显上调的LncRNAs,最后采用siRNA干扰的方法下调LncRNAs表达后分析其对成骨分化过程的影响。结果BMP-2诱导C2C12成骨分化过程中,成骨指标ALP、SPT增高,成肌指标MYOG降低。芯片结果表明,LncRNA AK051397在BMP2诱导后表达明显上调,处理组与未处理组相比升高4.9倍（P〈0.05）。干扰AK051397后成骨分化指标ALP、SP7表达下降,MYOG表达上升。结论LncRNA AK051397在C2C12细胞中具有促进成骨分化的作用,同时也具有抑制细胞成肌分化的作用。 |
| 关键词: 骨形态发生蛋白2 成骨分化 C2C12细胞 AK051397 |
| DOI：10.3969/j.issn1008-0392.2014.04.005 |
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| 基金项目:国家自然科学基金（81171178） |
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| Involvement of LncRNA AK051397 in BMP-2 induced-osteogenic differentiation of myoblast C2C12 cells |
| GAO Yan,CHENG Chen,LI Jing,XIAO Wei-fan,PAN Qiu-hui |
| (Dept. of Central Laboratory, Tenth People＇s Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To investigate the roles of LncRNA AK051397 in bone morphogenetic protein- 2 （BMP-2） induced-osteogenic differentiation of mouse myoblast C2C12 cells. Methods Osteogenic differentiation of C2C12 cells was induced by BMP-2 in vitro, the osteogenesis related indicators were examined by Real-Time PCR and ALP staining. The differential expression of LncRNAs during osteogenic differentiation in C2C12 cells was screened by ArrayStar LncRNA Array and the detected high-expressed LncRNAs were knocked-down by siRNA interference technology. Results RT-PCR and ALP staining revealed that the osteogenic differentiation indicators ALP and SP7 were increased, while myogenic differentiation indicator MYOG was decreased in C2C12 cells after BMP-2 treatment. LncRNA Array showed that compared to untreated C2C12 cells, the expression of LncRNA AK051397 was increased by 4.9-fold after BMP-2 treatment （P〈0.05）. The expression of ALP, SP7 was decreased and MYOG was increased in C2C12 cells after knock-down of LncRNA AK051397 by siRNA interference. Conclusion LncRNA AK051397 may be involved in osteogenic differentiation in C2C12 myoblast cells induced by BMP-2. |
| Key words: bone morphogenetic protein-2 BMP-2 osteogenic differentiation C2C12 cell AK051397 |
