| 引用本文： |
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赵莹莹,周秋莲,王菲,等.miRNA-21-3p对小鼠肝细胞系BNL CL.2体积和增殖的影响[J].同济大学学报（医学版）,2014,35(4):7-11. [点击复制]
- ZHAO Ying-ying1,ZHOU Qiu-lian2,WANG Fei1,et al.Effects of miRNA-21.3p on mouse hepatic cell line BNL CL. 2 cell size and proliferation[J].同济大学学报（医学版）,2014,35(4):7-11. [点击复制]
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| 摘要: |
| 目的观察miRNA-21-3p对小鼠肝细胞系（BNL CL.2）体积和增殖的影响。方法小鼠肝细胞系BNL CL.2转染miRNA-21-3p模拟物和抑制物。DiO染膜法和CCK-8法测定miRNA-21-3p对小鼠肝细胞系体积和增殖的影响;荧光定量PCR法检测miRNA-21-3p以及干预该miRNA后与细胞周期相关的基因的表达情况。结果在小鼠肝细胞系,转染miRNA-21-3p的模拟物可以显著增加miRNA-21-3p的表达,转染miRNA-21-3p抑制剂可以显著降低miRNA-21-3p的表达（P〈0.01）。干预miRNA-21-3p不影响细胞的体积。增加miRNA-21-3p的表达显著促进细胞增殖（P〈0.01）,而抑制miRNA-21-3p的表达可以显著降低细胞增殖（P〈0.01）。干预miRNA-21-3p,检测细胞周期相关的基因的表达,发现上调该miRNA可以下调Cyclin E（P〈0.05）,上调Ki67、p15、p16和p18（P〈0.05）,且对于CDKN3、Orc6、MCM4和p57没有影响（P〉0.05）。下调该miRNA对于以上基因均没有影响（P〉0.05）。结论miRNA-21-3p对于小鼠肝细胞系体积没有影响,但是可以调控增殖,且它对于增殖的调控效应是部分通过影响周期相关的基因实现。 |
| 关键词: miRNA-21-3p 肝细胞 增殖 |
| DOI：10.3969/j.issn1008-0392.2014.04.002 |
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| 基金项目:国家自然科学基金（81070343） |
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| Effects of miRNA-21.3p on mouse hepatic cell line BNL CL. 2 cell size and proliferation |
| ZHAO Ying-ying1,ZHOU Qiu-lian2,WANG Fei1,SONG Yang1,Yang Yue-feng1,YANG Chang-qing1 |
| (1. Dept. of Gastroenterology, Tongji Hospital, Tongji University, Shanghai 200065, China;;2. Regeneration Lab and Experimental Center of Life Sciences, Shanghai University, Shanghai 200444, China) |
| Abstract: |
| Objective To investigate the effects of microRNA-21-3p （miRNA-21-3p） on the cell size and proliferation of mouse hepatic cell line BNL CL. 2. Methods BNL CL. 2 cells were transfected with miRNA-21-3p mimics or inhibitor. Cell size was determined with DiO staining while cell proliferation was determined by CCK-8 assay. The expression level of miRNA-21-3p and cell cycle- related genes were detected by Real-Time PCR. Results In BNL CL. 2 cells, miRNA-21-3p mimics increased the expression of miRNA-21-3p, while miRNA-21-3p inhibitor decreased the expression （P〈0.01 ）. Although miRNA-21-3p mimics significant increased proliferation of BNL CL. 2 cells, the cell size was not changed;while miRNA-21-3p inhibitors suppressed cell proliferation, miRNA-21-3p mimics significantly down-regulated Cyclin E expression（P〈0.05 ） and up-regulated Ki67, p15, p16 and p18 （ P〈0.05 ） expression; while it did not affect CDKN3, Orc6, MCM4 and p57 expression （P〉0.05 ）. The expression of cell cycle-related genes in BNL CL. 2 cells was not changed after transfected with miRNA-21-3p inhibitors （P〉0.05 ）. Conclusion miRNA-21-3p promotes proliferation of mouse hepatic BNL CL. 2 cells without affecting cell size, which is associated with regulating some cell cycle- related genes expression. |
| Key words: miRNA-21-3p hepatocyte proliferation |
