| 引用本文： |
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陈婧,孙奋勇,马纪.miR-940慢病毒表达载体的构建以及对鼻咽癌细胞的影响[J].同济大学学报（医学版）,2014,35(3):1-4. [点击复制]
- CHEN Jing,SUN Fen-yong,MA Ji.Construction of recombinant lentivirus vector containing miR-940 and its effect on human nasopharyngeal carcinoma cells[J].同济大学学报（医学版）,2014,35(3):1-4. [点击复制]
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| 摘要: |
| 目的 构建microRNA-940(miR-940)的慢病毒表达栽体，制备重组慢病毒感染人鼻咽癌细胞5-8F,检测其对细胞迁移和增殖的影响。方法 构建慢病毒载体，包装后感染人鼻咽癌细胞，通过加药筛选方法获得稳定过表达miR-940的人鼻咽癌细胞株5-8F/miR-940。实时定量PCR(RT-PCR)检测miR-940的表达量。MTT测定miR-940对5-8F体外增殖的影响。划痕试验检测miR-940对5-8F迁移的影响。结果 构建稳定过表达miR-940的慢病毒表达栽体。MTT证明miR-940可以抑制5-8F在体外的增殖。划痕试验说明miR-940可以抑制5-8F的迁移能力。结论 miR-940能够抑制5-8F在体外的增殖以及迁移能力。 |
| 关键词: miR-940 鼻咽胂瘤 细胞迁移 细胞增殖 |
| DOI：10.3969/j.issn1008-0392.2014.03.001 |
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| 基金项目:国家自然科学基金（81301704) |
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| Construction of recombinant lentivirus vector containing miR-940 and its effect on human nasopharyngeal carcinoma cells |
| CHEN Jing1,SUN Fen-yong1,MA Ji2 |
| (1.Dept, of Clinical Laboratory, Tenth People’s Hospital, Tongji University, Shanghai 200072, China;2.Dept, of Central Laboratory, Tenth People’s Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To construct recombinant lentivirus vector containing miR-940, and to investigate its effect on human nasopharyngeal carcinoma cells. Methods Human nasopharyngeal carcinoma 5-8F cells were transfected with lentivirus vector containing miR-940. The expression of miR-940 in transfected 5-8F cells was detected by RT-PCR, the cell proliferation and migration were assayed by MTT test and wound healing assay, respectively. Results The lentivirus vector was constructed successfully. The transfected 5-8F cells expressed miR-940 stably. Cell proliferation and migration were suppressed in miR-940 transfected 5-8F cells. Conclusion miR-940 can inhibit proliferation and migration of 5-8F cells. |
| Key words: miR-940 nasopharyngeal carcinoma cells cell migration cell proliferation |
