| 引用本文： |
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李晓宇,罗欣枫,房林.miR-760靶向CDK8基因对人乳腺癌细胞 MDA-MB-231增殖与迁移的影响[J].同济大学学报（医学版）,2014,35(1):1-6. [点击复制]
- LI Xiao-yu,LUO Qi-feng,FANG Lin.miR-760 inhibits proliferation and migration by targeting CDKH in human breast cancer cell line MDA-MB-231[J].同济大学学报（医学版）,2014,35(1):1-6. [点击复制]
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| 摘要: |
| 目的探讨miR-760对人乳腺癌细胞中CDK8蛋白表达影响及其在乳腺癌细胞MDA-MB-231中的功能。 方法使用实时定量PCR方法检测miR-760在人乳腺癌细胞中的表达;使用脂质体介导的miRNA转染、Western 印迹法、MIT法、流式细胞仪技术分别检测转染miR-760前后CDK8蛋白表达差异及其对乳腺癌细胞增殖、迁移等 细胞功能和细胞周期的影响。结果人乳腺癌细胞中miR-760表达水平较正常乳腺细胞降低；外源性上调miR-760 可有效抑制人乳腺癌细胞株MDA-MB-231 CDK8蛋白表达（抑制效率为46.3%，P<0.05);人乳腺癌细胞株 MDA-MB-231转染miR-760后，其细胞增殖、迁移能力显著降低（P<0.05)。结论miR-760可能通过靶向CDK8 影响人乳腺癌细胞增殖与迁移，在乳腺肿瘤发生发展中起到抑癌基因作用。 |
| 关键词: 乳腺肿瘤；miR-760 CDX8基因 |
| DOI：10.3969/j.issnl008-0392.2014.01.001 |
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| 基金项目:国家自然科学基金（81272240) |
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| miR-760 inhibits proliferation and migration by targeting CDKH in human breast cancer cell line MDA-MB-231 |
| LI Xiao-yu,LUO Qi-feng,FANG Lin |
| (Dept. of Breast and Thyroid Surgery, Tenth People's Hospital, Tongji University, Shanghai 200072, China) |
| Abstract: |
| Objective To investigate the effects of miR-760 on CDK8 protein expression, cell proliferation and migration in human breast cancer MDA-MB-231 cell line.Methods The expression of miR-760 was quantitatively examined by Real-Time PCR in human breast cancer MDA-MB-231 cells and normal breast cells. miR-760 was transfected in MDA-MB-231 cells with lipofection ； the expression level of CDK8 protein，cell proliferation，cell cycle, cell migration of transfected MDA-MB-231 cells were assayed by Western blotting, MTT, flow cytometry and Transwell test, respectively. Results The expression level of miR-760 was significantly decreased in the breast cancer cells compared with normal breast cells. After transfection of miR-760 mimics, the capacities of breast cancer cell proliferation and migration were significantly reduced； and the expression of CDK8 protein was inhibited (P<0.05). Conclusion miR-760 can inhibit the cell proliferation and migration of human breast callcer MDA-MB-231 cells by targeting CDK8 |
| Key words: breast cancer miR-760 CDK gene |
