| 引用本文： |
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赵珍珍,李俊,王维刚,等.不同癌细胞系对斑蝥酸钠的药物敏感性检测[J].同济大学学报（医学版）,2013,34(4):10-15. [点击复制]
- ZHAO Zhen-zhen,LI Jun,WANG Wei-gang,et al.Drug susceptibility to sodium cantharidate of different cancer cell lines[J].同济大学学报（医学版）,2013,34(4):10-15. [点击复制]
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| 摘要: |
| 目的检测不同肿瘤细胞对斑蝥酸钠（sodium cantharidinate，SCA）药物敏感性的差异。方法培养14类肿瘤的22种细胞系，MTT法检测不同癌细胞对SCA的药物敏感性，流式细胞仪检测SCA对细胞周期和凋亡的影响。结果SCA对22种癌细胞系均有增殖抑制作用，且呈剂量依赖性。SCA能阻滞肿瘤细胞在G2／M期，抑制细胞增殖，诱导细胞凋亡，且作用呈现显著的剂量依赖关系。结论SCA作为一种广谱抗肿瘤药物，可能通过抑制细胞增殖，诱导细胞凋亡发挥作用。 |
| 关键词: 斑蝥酸钠 癌细胞系 药物敏感试验 增殖 凋亡 细胞周期 |
| DOI：10.3969/j. issnl008 -0392.2013.04.003 |
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| 基金项目:国家自然科学基金（81261120568） |
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| Drug susceptibility to sodium cantharidate of different cancer cell lines |
| ZHAO Zhen-zhen,LI Jun,WANG Wei-gang,MA Yu-shui,FEI Jian |
| (School of Life Science and Technology, Tongji University, Shanghai 200092, China; Shenqi Research Center for Ethnic Medicine, Tongji University, Shanghai 200092, China; 3. Shanghai Research Center for Model Organisms, Shanghai 201210, China);School of Life Science and Technology, Tongji University, Shanghai 200092, China; 2. Shenqi Research Center for Ethnic Medicine, Tongji University, Shanghai 200092, China; 3. Shanghai Research Center for Model Organisms, Shanghai 201210, China)) |
| Abstract: |
| Objective To test drug susceptibility to sodium cantharidinate （SCA） of different cancer cell lines. Methods Drug susceptibility to SCA was tested by MTT method in 22 cell lines from 14 cancer types. Cell cycle and apoptosis of cancer cell lines after SCA treatment were detected by flow cytometry. Results SCA inhibited the proliferation of all 22 cancer cell lines. And SCA blocked the cell cycle at G~/M phase and induced apoptosis of these cancer cells. Conclusion Results indicate that SCA may be used for cancer treatment. |
| Key words: sodium cantharidinate cancer cell lines drug susceptibility proliferation apoptosis cell cycle |
