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樊健,俞光荣,袁锋,等.Glut-1在良恶性骨肿瘤中的表达及临床意义[J].同济大学学报（医学版）,2013,34(3):28-31. [点击复制]
FAN Jian,YU Guang-rong,YUAN Feng,et al.Expression of glucose transporter protein-1 in benign and malignant bone tumors and its clinical significance[J].同济大学学报（医学版）,2013,34(3):28-31. [点击复制]

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Glut-1在良恶性骨肿瘤中的表达及临床意义
樊健¹,俞光荣¹,袁锋¹,陈凯¹,梅炯¹,易祥华²
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(1.同济大学附属同济医院骨科,上海200065;2.同济大学附属同济医院病理科,上海200065)

摘要:

目的探讨葡萄糖转运蛋白1(Glut-1)在人正常骨组织及良恶性骨肿瘤中的表达及临床意义。方法应用免疫组化检测32例骨肉瘤、10例骨软骨瘤及10例正常骨组织标本中Glut-1表达水平;应用Western-Blot检测3例新鲜骨肉瘤标本及正常骨组织中Glut-1的蛋白表达情况。结果免疫组化检验32例骨肉瘤标本阳性和强阳性31例(96.9%),其中强阳性20例(62.5%),仅有1例呈弱阳性;骨软骨瘤标本除1例为弱阳性其余都为阴性,正常骨组织中未见Glut-1阳性表达。West-blot显示3例新鲜骨肉瘤标本灰度比较正常骨组织明显增大(P<0.05)。结论Glut-1在骨肉瘤组织中呈高水平表达,可作为判断骨肉瘤恶性程度参考及可能的治疗靶点。

关键词: 骨肉瘤葡萄糖转运蛋白1 免疫组化

DOI：10.3969/j.issn1008-0392.2013.03.007

基金项目:

Expression of glucose transporter protein-1 in benign and malignant bone tumors and its clinical significance

FAN Jian¹,YU Guang-rong¹,YUAN Feng¹,CHEN Kai¹,MEI Jiong¹,YI Xiang-hua²

(1.Dept.of Orthopedics,Tongji Hospital,Tongji University,Shanghai 200065,China;2.Dept.of Pathology,Tongji Hospital,Tongji University,Shanghai 200065,China)

Abstract:

Objective To investigate the expression of glucose transporter protein-1（Glut-1） in normal bone tissue,benign and malignant bone tumors and its clinical significance.Methods Immunohistochemical staining was applied to detect Glut-1 expression in 32 cases of bone osteosarcoma,10 cases of osteochondroma and 10 specimens of normal bone tissue.The expression of Glut-1 protein was also examined by Wester-blotting method in fresh specimens of osteosarcoma and normal bone tissue.Results Immunohistochemistry showed positive expression of Glut-1 in 31cases of osteosarcoma（96.9%）,including strongly positive in 20 cases（62.5%） and weakly positive in 1 case.All osteochondroma specimens presented negative staining for Glut-1,except one case presenting weakly positive,while no positive expression of Glut-1 was detected in normal bone tissue.Western-blot showed the gray value increased significantly in 3 fresh specimens of osteosarcoma compared to normal bone tissue（P＜0.05）.Conclusion Glut-1 protein is positively expressed in osteosarcoma,which indicates that Glut-1 may be used to assess malignancy of bone tumors and as a potential target for treatment of bone tumors.

Key words: bone sarcoma Glucose transporter protein-1 immunohistochemistry