| 摘要: |
| 目的 建立乙酰胆碱诱导的离体Langendorff灌流的兔的心房颤动模型。方法 健康雄性的新西兰兔40只,2.2~2.5kg分为4组,分别为正常灌流组、正常灌流+burst刺激组、正常灌流+乙酰胆碱灌注组和正常灌流+乙酰胆碱灌注+burst刺激组,每组各10只。在Langendorff灌流装置下行主动脉逆行灌流,通过乙酰胆碱灌注和burst刺激诱发心房颤动。结果 正常灌流组、正常灌流+burst刺激组和正常灌流+乙酰胆碱灌注组均没有发生心房颤动,而正常灌流+乙酰胆碱灌注+burst刺激组心房颤动的发生率为50%。结论 在Langendorff心脏灌流时,给予乙酰胆碱灌注模拟胆碱能激活,并给予burst刺激模拟应激事件,可以稳定地诱导心房颤动的发生。 |
| 关键词: 心房颤动 乙酰胆碱 新西兰兔 |
| DOI：10.3969/j.issn1008-0392.2012.03.008 |
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| 基金项目:国家自然科学基金，上海市自然科学基金项目 |
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| Establishment of acetylcholine-induced atrial fibrillation model in isolated rabbit heart |
| PAN Lei1,2,LI Jun1,2 |
| (1.Oriental Medical Translational Research Center of Tongji University,Shanghai 200120,China; 2.Key Laboratory of Arrhythmias Studies,Ministry of Education at Tongji University,Shanghai 200120,China) |
| Abstract: |
| Objective To establish an acetylcholine-induced atrial fibrillation(AF)model in the isolated rabbit heart.Methods Forty healthy male New Zealand rabbits(2.2~2.5kg)were used in the study.The isolated rabbit hearts were randomly assigned to normal perfusion(group A),normal perfusion with burst high-frequency electric stimulation(group B),acetylcholine perfusion(group C) and acetylcholine perfusion with burst electric stimulation(group D)(n=10 in each group).The isolated hearts were perfused with Langendorff perfusion system.Results The AF incidence in group D(acetylcholine perfusion combined with burst electric stimulation)was 50%;while no AF was induced in other three groups.Conclusion A simple and stable acetylcholine-induced atrial fibrillation model has been established in isolated rabbit heart with Langendorff perfusion system. |
| Key words: atrial fibrillation acetylcholine New Zealand rabbit |
