| 引用本文： |
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李瑾,李微,殷少军,等.活性维生素D3抑制大鼠间质成纤维细胞激活作用的研究[J].同济大学学报（医学版）,2012,33(2):38-42. [点击复制]
- LI Jin,LI Wei,YIN Shao-jun,et al.1,25-dihydroxyvitamin D3 suppresses fibronectin production induced by TGF-β1 in renal interstitial fibroblasts[J].同济大学学报（医学版）,2012,33(2):38-42. [点击复制]
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| 摘要: |
| 目的通过观察活性维生素D3(1,25-(OH)2VitD3,活性VitD3)抑制转化生长因子β1(Transforming growthfactorβ1,TGF-β1)诱导大鼠肾脏间质成纤维细胞(NRK-49F)合成纤维连接蛋白(Fibronectin,FN)作用的研究,探讨活性VitD3阻止肾组织纤维化作用的机制。方法体外培养的NRK-49F细胞,分为对照组、不同时间TGF-β1处理组、活性VitD3处理组、不同剂量活性VitD3与TGF-β1联合处理组。通过蛋白免疫印迹、免疫荧光染色等实验方法,分别以α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)作为成纤维细胞被激活指标、FN作为细胞外基质合成的检测指标,比较不同浓度活性VitD3对经TGF-β1刺激的NRK-49F细胞表达α-SMA以及合成FN的变化。结果TGF-β1可以诱导NRK-49F的α-SMA和FN蛋白表达明显增加,并呈一定的时间依赖性,同时,还可影响α-SMA和FN蛋白结构的组装和沉积;活性VitD3单独作用对α-SMA和FN蛋白表达和组装无明显影响,而活性VitD3与TGF-β1同时作用于NRK-49F时,活性VitD3可以部分拮抗TGF-β1的效应,抑制α-SMA和FN蛋白表达,并呈剂量依赖关系。结论活性VitD3可以部分拮抗TGF-β1诱导的肾成纤维细胞的活化,抑制细胞外基质FN的合成分泌。 |
| 关键词: 活性维生素D3 成纤维细胞 转化生长因子β1 纤维连接蛋白 |
| DOI：10.3969/j.issn1008-0392.2012.02.008 |
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| 基金项目: |
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| 1,25-dihydroxyvitamin D3 suppresses fibronectin production induced by TGF-β1 in renal interstitial fibroblasts |
| LI Jin,LI Wei,YIN Shao-jun,CHEN Wei-jie |
| (Dept.of Gereology,Tenth People’s Hospital,Tongji University,Shanghai 200072,China) |
| Abstract: |
| Objective To investigate the effect of 1,25-(OH)2 VitD3 on the production of fibronectin induced by transforming growth factor β1(TGF-β1) in renal interstitial fibroblasts.Methods To study the effect of 1,25-(OH)2 VitD3 and TGF-β1 on ECM synthesis.The renal interstitial fibroblast NRK-49F cells were divided into control group,TGF-β1 group,VitD3 group and TGF-β1 plus VitD3 group.The de novo expression of α-smooth muscle actin(α-SMA) and fibronectin were detected by western blot analysis and immunofluorescence.Results TGF-β1 induced de novo expression of α-SMA and production of fibronection in a time-dependent manner.TGF-β1 also affected assembly of α-SMA and deposition of fibronectin.1,25-(OH)2 VitD3 alone had no effects on α-SMA and fibronectin expression.Whereas,1,25-(OH)2VitD3 portially obliterated T expression of α-SMA and fibronectin in a dose-dependent manner.Conclusion The results indicate that 1,25-(OH)2 VitD3 can partially inhibit renal interstitial fibroblast activation and fibronectin synthesis induced by TGF-β1. |
| Key words: 1,25-(OH)2VitD3 fibroblast transforming growth factor β1 fibronectin |
