MA Tian-rui,HAN Jie,CHEN Hai-yan,et al.Reduced apoptosis of CD4+T cells and expression of apoptosis-related signal proteins in patients with rheumatoid arthritis[J].同济大学学报（医学版）,2011,32(5):36-40. [点击复制]
Objective To investigate the apoptosis of CD4+T cells and the expression of apoptosis-related signal proteins in patients with rheumatoid arthritis(RA).Methods The expression levels of Annexin V,Caspase-3,Caspase-8,Fas and Bcl-2 of CD4+T lymphocytes in patients with RA and control individuals were measured by using flow cytometry.The correlation between the expression of apoptosis signaling proteins and clinical activity parameters ESR,CPR,RF and DAS28 were analyzed. Results The level of CD4+T lymphocytes in RA patients was significantly higher than that in control individuals[(40.91±2.73)%vs(31.40±2.71)%,P＜0.05],while the value of AnnexinⅤ/ CD4+T was lower[(2.27±1.43)%vs(8.10±2.06)%,P＜0.05]that of control individuals. Compared with the control individuals,the expression rate of Fas in CD4+T cells of RA patients was significantly decreased in the active phases[(63.12±4.61)%vs(84.13±1.72)%,P＜0.01],the expression of Bcl-2 was significantly increased[(93.58±2.36)%vs(75.14±5.70)%,P＜0.05], the expression of Caspase-8 was decreased[(18.54±15.23)%vs(3.02±1.39)%,P＜0.05],but there was no statistical significance in Caspase3 expression between two groups[(2.17±1.19)%vs (2.53±1.46)%,P＞0.05].The expression of Fas on CD4+T cells was negatively correlated with DAS28 parameter(r=-0.89,P=0.04,P＜0.05),whereas Bcl-2 was positively correlated with DAS28 parameter(r=-0.97,P=0.00,P＜0.01).Conclusion In RA patients,the increased expression of CD4+T cells and the decreased expression of AnnexinⅤmight be associated with the decreased apoptosis rate,which may be involved in the pathogenesis of RA.The expression of Fas and Bcl-2 in CD4+T cells of RA patients might be a sensitive indicator for the clinical activity of RA.
