| 引用本文： |
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唐嘉佑,施向挺,王跃闽,等.逼尿肌无力尿失禁动物模型的建立[J].同济大学学报（医学版）,2011,32(5):16-19. [点击复制]
- TANG Jia-you,SHI Xiang-ting,WANG Yue-min,et al.Establishment of a rat model for detrusor atonyoccui urinary incontinence[J].同济大学学报（医学版）,2011,32(5):16-19. [点击复制]
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| 摘要: |
| 目的 探讨液氮冷冻膀胱壁建立膀胱逼尿肌无力尿失禁动物模型的可行性。方法 20只雌性成年未生产过的Wistar大鼠,随机分为实验组和对照组（n=10）。实验组开腹暴露膀胱后用置于-80℃液氮中的冷探针冷冻膀胱壁,对照组行假手术单纯开腹暴露膀胱。继续饲养2周后行离体逼尿肌收缩功能测试并观察膀胱壁病理变化。结果 分别比较对照组和实验组的逼尿肌最大张力和收缩频率测量结果,两者差异有统计学意义（P＜0.001）。膀胱逼尿肌组织学检查比较显示实验组肌纤维细而疏松,对照组肌纤维粗而紧密。结论 液氮冷冻膀胱壁可以建立膀胱逼尿肌无力尿失禁的动物模型,该模型发生率高、稳定。 |
| 关键词: 尿失禁 动物模型 液氮冷冻 大鼠 |
| DOI：10.3969/j.issn1008-0392.2011.05.004 |
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| 基金项目:国家自然科学基金(30571866) |
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| Establishment of a rat model for detrusor atonyoccui urinary incontinence |
| TANG Jia-you1,SHI Xiang-ting2,WANG Yue-min2,Fan Hui-min1,LIU Zhong-min1 |
| (1.Institute of Transplantation and Immunology,Research Center for Translational Medicine, Tongji University,Shanghai 200120,China;2.Dept.of Urology,East Hospital, Tongji University,Shanghai 200120,China) |
| Abstract: |
| Objective To establish an animal model for stress urinary incontinence in rat by freezing the bladder wall with liquid nitrogen.Methods Twenty adult female Wistar rats were divided into experimental group(n=10) and the control group(n=10).In experimental group the bladder of rats was exposed after laparotomy and bladder wall was frozen with a cold probe pretreated with liquid nitrogen (-80℃),the rats in control group were sham-exposed with simple laparotomy.Two weeks after operation the contraction of isolated detrusor was tested and the pathological changes in the bladder wall were observed.Results There were significant differences in the maximum tension and contraction of the detrusor frequency between control group and experimental group(P＜0.001).Histological examination of bladder detrusor shows that the muscle fibers in experimental group were loose and thin,however,those in control group were thick and tight.Conclusion Freezing the bladder wall with liquid nitrogen can establish animal model of bladder detrusor weakness of urinary incontinence of high incidence and stable. |
| Key words: urinary incontinence animal model liquid nitrogen freezing rat |
