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王惠英,江志坚,吴罕樑,等.早期糖尿病大鼠视网膜形态变化及相关因子分析[J].同济大学学报（医学版）,2011,32(3):15-19. [点击复制]
WANG Hui-ying,JIANG Zhi-jian,WU Han-liang,et al.Retinal morphologic changes and related factors at early stage of diabetic rats[J].同济大学学报（医学版）,2011,32(3):15-19. [点击复制]

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早期糖尿病大鼠视网膜形态变化及相关因子分析
王惠英¹,江志坚²,吴罕樑³,董健鸿²,荣翱¹
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(1. 同济大学附属同济医院眼科,上海200065;2. 上海市徐汇区中心医院眼科,上海200040;3. 上海邮电医院眼科,上海200020)

摘要:

目的探讨早期糖尿病视网膜病变（diabetic retinopafhy，DR）大鼠的视网膜形态变化与血清血管内皮生长因子（Vascular endotllelial growm factor，VEGF）、内皮素（endomelin，ET）和一氧化氮（N0）的相关关系。方法 40只健康SD大鼠随机分为实验组和对照组，实验组腹腔注射链脲佐菌素建模成功后，按病程分为糖尿病2、4、6、8个月组；对照组腹腔注射等体积缓冲液，根据与实验组年龄匹配原则平均分为4组，每组5只。分别在第2、4、6、8个月检测血清VEGF、ET、NO含量，摘除眼球观察视网膜组织形态，应用sPss13．0进行统计学分析。结果实验组病程4个月始出现明显视网膜组织水肿，细胞排列紊乱，随病程延长逐渐加重。各实验组血清VEGF、ET含量较同鼠龄对照组高（P＜0．05），糖尿病2个月组血清No含量较同鼠龄对照组高（P＜0．05），而糖尿病6个月组及糖尿病8个月组血清N0含量较同鼠龄对照组低（P＜0．05）。随着病程延长，实验组血清VEGF、ET含量逐渐升高，N0含量逐渐降低（P＜0．05）。实验组血清VEGF与ET含量成正相关（r=0．821，P＜0．01），与N0含量成负相关（r=-0．814，P〈0．01）；血清ET含量与N0成负相关（r=-0．802，P＜0．01）。结论 VEGF、ET和N0的含量与DR严重程度存在相关性，考虑血清VEGF、ET和N0含量可以间接反映DR大鼠视网膜病变的严重程度。

关键词: 糖尿病视网膜病变血管内皮生长因子内皮素一氧化氮大鼠

DOI：10.3969/j.issn1008-0392.2011.03.004

基金项目:上海市科委科研基金(064119614)

Retinal morphologic changes and related factors at early stage of diabetic rats

WANG Hui-ying¹,JIANG Zhi-jian²,WU Han-liang³,DONG Jian-hong²,RONG Ao¹

(1.Dept.of Ophthalmology,Tongji Hospital,Tongji University,Shanghai 200065,China;2.Dept.of Ophthalmology,Xu Hui District Central Hospital,Shanghai 200040,China;3.Dept.of Ophthalmology,Shanghai Posts And Communications Hospital,Shanghai 200020,China)

Abstract:

Objective To investigate the correlation between the retinal morphologic changes and the levels of vascular endothelial growth factor(VEGF),endothelin(ET) and nitric oxide(NO) in rats with diabetic retinopathy(DR).Methods Forty SD rats were randomly assigned into the experimental group and the control group.To establish the DR model,rats in experimental group were intraperitoneally injected with streptozotocin(60 mg/kg).Rats in control group were intraperitoneally injected with the same volume of buffer.Rats in both groups were randomly assigned into four subgroups with 5 rats in each subgroup.The animals were observed at 2,4,6 and 8 months respectively after the establishment of DR model.The serum levels of VEGF,ET and NO were measured,the eyeballs enucleated and the retinal morpha observed.The data were analyzed with SPSS 13.0 software.Results In the experimental group, retinal edema and cellular disorganization appeared at 4 months and became more severe when diabetes course extended.The serum levels of VEGF and ET in the experimental group were significantly higher than those in the control group(P＜0.05).The level of serum NO in 2-month experimental subgroup was significantly higher than that in the corresponding control subgroup(P＜0.05).The level of serum NO in 6- and 8-month experimental subgroups was significandy lower than that in corresponding control subgroups (P＜0.05).With the progression of the disease,serum VEGF and ET levels increased gradually and the NO level decreased gradually(P＜0.05).In the experimental group,the VEGF level was positively correlated with that of ET(r =0.821,P＜0.01) but negatively correlated with that of NO(r= -0.814 & r=-0.802,P＜0.01).Conclusion The levels of serum VEGF,ET and NO are correlated with the seriousness of DR and may predict the DR development indirectly.

Key words: diabetic retinopathy vascular endothelial growth factor endothelin nitric oxide rats