SHEN Zhen-yu,JIANG Xiao-feng,SUN Bin,et al.Study on protective mechanism of reduced glutathione on patients with acute pancreatitis-associated liver injury[J].同济大学学报（医学版）,2011,32(1):73-76. [点击复制]
Objective To study the protective mechanism of reduced glutathione on patients with acute pancreatitis-associated liver injury.Methods Eighty-four patients with liver injury and acute pancreatitis were randomly divided into two groups: routine treatment group(n=42) and GSH treatment group(n=42).Liver function tests,TNF-α,IL-6,IL-10,malondialdehyde(MDA),glutathione peroxidese(GSH-Px)and superoxide dismutase(SOD)were assessed on admission and on the 8th day.Results Compared with the routine treatment,GSH expressed better efficiency in the treatment of patients with acute pancreatitis-associated liver injury(P＜0.05).The serum levels of TNF-α[(2.34±0.15)/ng·ml-1] and IL-6[(144.52±10.75)pg·ml-1] after GSH treatment were significantly lower than those in routine treatment group[(4.30±0.26)/ng·ml-1;(244.82±15.16)/pg·ml-1,(P=0.001)] respectively.While IL-10(71.57±3.07)pg·ml-1 in serum in GSH treatment group was significantly higher than that in routine treatment group[(65.98±3.17)pg·ml-1,(P=0.002 4),the concentrations of TNF-α and IL-6 after GSH treatment were significantly lower than those in routine treatment group[(4.41±0.25)ng·ml-1;(246.62±14.78)pg·ml-1,(P=0.001)] respectively.The serum level of IL-10 after GSH treatmentwas significantly higher than that in routine treatment group(67.22±3.25)pg·ml-1,(P=0.003 6).The serum MDA(40.08±1.92)μmol·L-1and SOD[(19.75±1.23)KU·L-1] levels after GSH treatment were significantly lower than those in routine treatment group(44.07±1.98)μmol·L-1;(24.52±1.21)KU·L-1,(P=0.003)和(P=0.001) respectively.However,the serum level of GSH-Px(48.13±1.85)μg·L-1 in GSH treatment groupwas significantly higher than that in routine treatment group[(41.19±1.80)μg·L-1;P=0.001)].The concentrations of MDA and SOD after GSH treatment were significantly lower than those in routine treatment group[(42.37±1.94)μmol·L-1,(24.14±1.31)KU·L-1;(P=0.003,P=0.001)] respectively.Compared with control group[(40.48±1.48)μg·L-1],the serum level of GSH-Pxwas significantly higher in GSH treatment group(P=0.001).Conclusion Reduced glutathione has beneficial effects on the treatment of liver injury with acute pancreatitis.The modulating effects of the inflammatory medi a and oxygen free radical may be involved in this mechanism.
