| 引用本文： |
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吴萍,许树长,孔炫,等.Ghrelin在反流性食管炎患者胃底组织中的表达[J].同济大学学报（医学版）,2011,32(1):51-55. [点击复制]
- WU Ping,XU Shu-chang,KONG Xuan,et al.Expression of Ghrelin in fundus of stomach in patients with reflux esophagitis[J].同济大学学报（医学版）,2011,32(1):51-55. [点击复制]
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| 摘要: |
| 目的 测定胃底黏膜组织内Ghrelin的含量,研究Ghrelin与反流性食管炎的关系。方法 收集上海同济大学附属同济医院2009年8月—2009年11月消化内科门诊患者,体质量指数（body mass index,BMI）在18.5~23.9 kg/m2,按洛杉矶标准胃镜确诊为反流性食管炎（reflux esophagitis,RE）且用快速尿素酶试验检测幽门螺杆菌（Helicobacter pylori,Hp）均为阴性患者,其中A级14例,B级6例,另收集性别构成、年龄、BMI与RE组匹配的体检患者20例（病理改变仅为轻度浅表性胃窦炎且用快速尿素酶试验检测Hp均为阴性）设为正常对照组,二组均在胃镜下取胃底黏膜一块,应用实时荧光定量（Real-Time qualitative）PCR法测定Ghrelin的相对含量并比较二组结果,同时比较A级和B级RE患者胃底黏膜内Ghrelin的相对含量。结果 RE组和正常对照组比较,Ghrelin的表达有统计学差异（P＜0.05）;A级和B级RE患者比较,Ghrelin的表达无统计学差异（P＞0.05）。结论 Ghrelin促发一过性食管下括约肌松弛,使胃食管酸暴露增加,可能在反流性食管炎的发病机制中发挥一定的作用。 |
| 关键词: 反流性食管炎 Ghrelin 实时荧光定量PCR |
| DOI：10.3969/j.issn1008-0392.2011.01.051 |
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| 基金项目: |
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| Expression of Ghrelin in fundus of stomach in patients with reflux esophagitis |
| WU Ping1,XU Shu-chang2,KONG Xuan3,LIU Yuan-fei2 |
| (1.Dept.of Clinical Nutrition,Tongji Hospital,Tongji University,Shanghai200065,China;2.Dept.of Gastroenterology，Tongji Hospital，Tongji University，Shanghai 200065，China;3.Dept.of Gastroenterology,Central Hospital of Yangpu District,Shanghai 200093,China) |
| Abstract: |
| Objective To study the relationship between Ghrelin and reflux esophagitis(RE) by detecting the quantity of Ghrelin in gastric mucosa.Methods Included in this study were twenty gastroenterology outpatients diagnosed RE from Aug.to Nov.2009 by endoscopy inspection according to Los Angeles endoscopy standard were.Their body mass indexes(BMI) were between 18.5kg/m2 and 23.9kg/m2,and their Helicobacter pylori(Hp) were negative by the rapid urease test.Among them fourteen patients were A grade and six patients were B grade . Twenty healthy subjects whose gender composition, age and BMI matched with the RE group served as controls with negative Hp too. Gastro-duodenal endoscopy was performed with all subjects drawn a mucosa in the stomach antrum and the relative quantity of Ghrelin measured then by Real-Time fluorescence quantitative polymerase chain reaction. Results of RE and control groups and the results of A and B grade groups were compared. Results The relative quantity of Ghrelin in the mucosa with RE was significantly lower than that in healthy controls (P＜0.05) , while Ghrelin had no statistical difference between A and B grade patients (P＞0.05). Conclusion Ghrelin precipitats the transient lower esophageal sphincter relaxations and increases the gastro-esophageal acid exposure. Hence it may play a role in the pathogenesis of RE. |
| Key words: reflux esophagitis Ghrelin Real-Time quantitative RCR |
