引用本文: |
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周健红,吴帆,成佳景,等.夫妇HLA-DR抗原相容性与习惯性流产的关系[J].同济大学学报(医学版),2010,31(6):98-101. [点击复制]
- ZHOU Jian-hong,WU Fan,CHENG Jia-jing,et al.Relationship between couple's compatibility of HLA-DR and recurrent spontaneous abortion[J].同济大学学报(医学版),2010,31(6):98-101. [点击复制]
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摘要: |
目的探讨夫妇HLA-DR抗原相容性和习惯性流产的关系。方法采集2007年8月—2009年8月在我院就诊的30对习惯性流产夫妇外周血,各5 ml,以同时期正常妊娠夫妇作为对照组,抽提样本基因组DNA,确定HLA-DR等位基因位点及其对应抗原,分析疾病组与对照组中抗原相容性分布是否具有差异。结果在检出的DR抗原中,两组抗原频率差别无统计学意义(P〉0.05)。疾病组中,有6对(20%)夫妇HLA-DR抗原零相容,18对(60%)夫妇一个抗原相容,6对(20%)夫妇两个抗原相容。对照组中,21对(70%)夫妇零相容,7对(23%)夫妇一个抗原相容,2对(7%)夫妇两个抗原相容。两组夫妇抗原相容性分布有显著性差异(P〈0.01)。一个抗原相容对于零抗原相容的相对危险度(RR)为9,两个抗原相容相对于零抗原相容的相对危险度(RR)为10.5。结论习惯性流产的发生与免疫失衡有关,夫妇双方HLA-DR抗原相容性增加,致母体对异体抗原识别不足,不能产生足够的封闭性抗体,胚胎遭排斥而产生流产。 |
关键词: 习惯性流产 HLA-DR抗原 序列特异性引物聚合酶链式反应 相容性 移植排斥 |
DOI:10.3969/j. issnl008 - 0392.2010.06.024 |
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基金项目: |
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Relationship between couple's compatibility of HLA-DR and recurrent spontaneous abortion |
ZHOU Jian-hong,WU Fan,CHENG Jia-jing,QIU Jin,Lei Jia,WU Yi |
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Abstract: |
Objective To study the relationship between couple's compatibility of human leukocyte classⅡDR antigens(HLA-DR) and recurrent spontaneous abortion.Methods Thirty women with recurrent spontaneous abortion and 30 healthy pregnant women and their partners were enrolled from August 2007 to August 2009.Aliquots of 5 ml venous blood samples were taken in ethylenediamine tetraacetic acid solution tubes.Genomic DNA was extracted,then HLA-DR gene and antigen were typed and analyzed between two groups.Results HLA-DR typing did not show any particular antigen clearly associated with recurrent spontaneous abortion(P0.05).In study group,6 couples(20%) had zero compatible HLA-DR antigen,18 couples(60%) had one compatible HLA-DR antigen,6 couples(20%) had two compatible HLA-DR antigens.In control group,21 couples(70%) had zero compatible HLA-DR antigen,7 couples(23%) had one compatible HLA-DR antigen,2 couples(7%) had two compatible HLA-DR antigens(P0.01).The antigen compatibility in study group was higher than controls.The relative risk of one compatible HLA-DR antigen was 9,the relative risk of two compatible HLA-DR antigens was 10.5.Conclusion Increased HLA-DR antigen compatibility between father and mother might contribute to recurrent spontaneous abortion as a result of an impaired ability to generate a protective maternal immune response.It leads to the failure of the mother's nonresponsiveness to fetal antigen.Lack of blocking factor increases the risk for recurrent spontaneous abortion. |
Key words: recurrent spontaneous abortion HLA-DR SSP-PCR compatibility transplant rejection |