| 引用本文： |
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余莹,李长明,周沛然,等.坎地沙坦在炎症过程中抑制氧化应激反应机制的研究[J].同济大学学报（医学版）,2010,31(3):59-63. [点击复制]
- YU Ying,LI Chang-ming,ZHOU Pei-ran,et al.The mechanism of anti-inflammation effects by candesartan during inflammation[J].同济大学学报（医学版）,2010,31(3):59-63. [点击复制]
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| 摘要: |
| 目的探讨坎地沙坦（Cande sartan,CAN）是否具有不依赖血管紧张素1型受体（anti-inflammation via independent,AT1R）的抗炎作用及其可能的机制。方法以人肾小管上皮细胞为研究对象，观察CAN对肿瘤坏死因子（TNF-α）诱导炎症趋化因子的影响及CAN对TNF-α所诱导ROS的作用。采用RT-PCR方法测定相关分子mRNA表达，Western blotting和ELISA方法测定相关分子蛋白含量。结果CAN能有效抑制TNF-α诱导炎症趋化因子MCP-1、RANTES mRNA和蛋白表达，且能抑制TNF-α诱导的NF-κB磷酸化。CAN具有类是抗氧化应激物N乙酰半胱氨酸（NCA）的作用．抑制TNF-α诱导的ROS产生。结论CAN能抑制TNF-α诱导肾小管上皮细胞发生炎症反应，其机制与CAN的直接抗氧化作用有关。 |
| 关键词: 血管紧张素Ⅱ1型受体 坎地沙坦 肿瘤坏死因子 趋化因子 活性氧 |
| DOI：10.3969/j .issn1008 -0392.2010.03.014 |
| 投稿时间：2010-03-06 |
| 基金项目:国家自然科学基金资助项目(3087 1 181)上海市浦'江人才计划(09PJ1408600)；上海市浦东新区科技发展基金创新资金(PKJ2008-Y 1 1 ) |
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| The mechanism of anti-inflammation effects by candesartan during inflammation |
| YU Ying1,LI Chang-ming1,ZHOU Pei-ran1,FU Lan-jun1,Lance Dworkin2,YU Chen1 |
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| Abstract: |
| Objective To study the role of Candesartan （CAN） in anti-inflammation via independent of AT1R pathway and its mechanism. Methods Renal tubular epithelial cells were stimulated by TNF-α. The effects of CAN on inflammatory chemokines and ROS synthesis in the cells induced by TNF-α were observed. The expression level of mRNA was detected by RT-PCR, and the levels of proteins were detected by Western blotting and ELISA. Results CAN suppressed mRNA and protein expressions of pro-inflammatory chemokines MCP-1 and RANTES in human proximal epithelial cells induced by TNF-α with a dose dependent fashion. CAN also inhibited TNF-α-induced NF-KB phosphorylation in the cells. CAN likes N-acetyl cysteine （NAC）, a reactive oxygen species （ROS） scavenger, blocked TNF-α and H202-induced ROS generation. Conclusion CAN can suppress infl＋ ammatory reponsiveness in the cell induced by TNF-α. The mechanism of the effect maybe associate with its direct anti-ROS generation. |
| Key words: angiotensin Ⅱ type 1 receptor Candesartan TNF-α chemokines reactive oxygenspecies （ROS） |
