| 引用本文： |
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倪艳艳,范跃祖.两种结肠癌动物模型的淋巴管生成比较[J].同济大学学报（医学版）,2010,31(3):19-22. [点击复制]
- NI Yan-yan,FAN Yue-zu.Comparison of lymphangiogenesis between subcutaneous xenograft model and in situ model of colon carcinomas[J].同济大学学报（医学版）,2010,31(3):19-22. [点击复制]
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| 摘要: |
| Objective To explore the possibility of establishing lymphangiogenetic model of human colon carcinoma in nude mice. Methods Subcutaneous xenograft model and in situ model of lymphangiogenesis of human colon carcinomas were established by transplanting subcutaneously or through in situ growth of human colon carcinoma cell line HT-29 in nude mice. The numbers of lymphangiogenetic microvessel were identified with lyve-1, ck20 by immunohistochemical staining. The expression levels of vascular endothelial growth factors （VEGF-C,VEGF-D and VEGFR-3） were measured by quantitative RT-PCR. Results The expression of VEGF-C, VEGF-D and VEGFR-3 mRNA levels were significantly higher in both subcutaneous tumors and in-situ cultured tumors than that of the negative control group （both P〈0.01）, and the expression differences were observed between in situ tumors and subcutaneous tumors （P〈0.05）. Moreover, lyve-1 and CK20- positive staining of lymphatic vessels were more intensive in in-situ tumors than that in subcutaneous tumors. Conclusion Either in situ or subcutaneous xenografted tumors of human colon carcinoma shows lymphangiogenesis, but the number and shape of lymphangiogenesis from in situ tumors are excessively. It may provide an ideal, referential animal model for further studying of the mechanism of lymph node metastasis mechanism, drug intervention and anti- metastasis therapy in colorectal cancer. |
| 关键词: 结肠肿瘤 动物模型 淋巴管生成 |
| DOI：10.3969/j. i s sn1008 -0392.2010.03 .005 |
| 投稿时间：2010-01-26 |
| 基金项目:上海市卫生局基金资助项目（034109) |
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| Comparison of lymphangiogenesis between subcutaneous xenograft model and in situ model of colon carcinomas |
| NI Yan-yan,FAN Yue-zu |
| () |
| Abstract: |
| Objective To explore the possibility of establishing lymphangiogenetic model of human colon carcinoma in nude mice. Methods Subcutaneous xenograft model and in situ model of lymphangiogenesis of human colon carcinomas were established by transplanting subcutaneously or through in situ growth of human colon carcinoma cell line HT-29 in nude mice. The numbers of lymphangiogenetic microvessel were identified with lyve-1, ck20 by immunohistochemical staining. The expression levels of vascular endothelial growth factors （VEGF-C,VEGF-D and VEGFR-3） were measured by quantitative RT-PCR. Results The expression of VEGF-C, VEGF-D and VEGFR-3 mRNA levels were significantly higher in both subcutaneous tumors and in-situ cultured tumors than that of the negative control group （both P〈0.01）, and the expression differences were observed between in situ tumors and subcutaneous tumors （P〈0.05）. Moreover, lyve-1 and CK20- positive staining of lymphatic vessels were more intensive in in-situ tumors than that in subcutaneous tumors. Conclusion Either in situ or subcutaneous xenografted tumors of human colon carcinoma shows lymphangiogenesis, but the number and shape of lymphangiogenesis from in situ tumors are excessively. It may provide an ideal, referential animal model for further studying of the mechanism of lymph node metastasis mechanism, drug intervention and anti- metastasis therapy in colorectal cancer. |
| Key words: colonic neoplasm animal models lymphangiogenesis |
