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李发洁,梁丹丹,肖俊杰,等.抑制线粒体苯二氮卓类受体对缺血心肌细胞APD90和ERP的影响[J].同济大学学报（医学版）,2010,31(1):23-26. [点击复制]
LI Fa-jie,LIANG Dan-dan,XIAO Jun-jie,et al.Effects of mBzR inhibition on APD90 and EEP of ischemic cardiomyocytes in rat[J].同济大学学报（医学版）,2010,31(1):23-26. [点击复制]

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抑制线粒体苯二氮卓类受体对缺血心肌细胞APD90和ERP的影响
李发洁,梁丹丹,肖俊杰,刘懿,汪世龙,陈义汉
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(同济大学心律失常教育部重点实验室,上海,200092;同济大学生命科学与技术学院,上海,200092 ;同济大学心律失常教育部重点实验室,上海,200092;同济大学医学遗传研究所,上海,200065;同济大学心律失常教育部重点实验室,上海,200092;同济大学附属同济医院心血管内科,上海,200065;同济大学心律失常教育部重点实验室,上海,200092;同济大学生命科学与技术学院,上海,200092;同济大学附属同济医院心血管内科,上海,200065;同济大学医学遗传研究所,上海,200065)

摘要:

目的研究抑制线粒体苯二氮卓类体（mitochondrial benzodiazepine receptor, mBzR）对缺血大鼠乳头肌动作电位复极至90％的时间（90％ofduration of action potential，APDgo）和有效不应期（effective refractory period．ERP）的影响。方法建立体外大鼠乳头肌模拟心肌缺血模型，将50只大鼠随机分为正常对照组、单纯缺血组、4’-chlorodiazepam（16μmol／L）组、4’-chlorodiazepam（32μmol/L）组和4’-chlorodiazepam（64μmol／L）组，每组各10只。利用细胞内标准玻璃微电极技术，记录APD。和ERP的变化。结果与正常对照组相比，缺血组APD。和ERP显著减小（P〈0．05）。缺血加4’-chlorodiazepam的各组都可以显著减少缺血时APDg）和ERP的降低程度（P〈0．05）。结论缺血时，心肌细胞APD。和ERP逐渐缩短，这可能是缺血促进折返性心律失常的基础之一：而线粒体mBzR受体特异性的抑制剂4＇-chlorodiazepam可以显著地减少缺血所致的APD。和ERP的缩短，从而减少了折返形成的可能性，这可能是抑制线粒体mBzR受体阻止心室颤动发生的重要的电生理基础。

关键词: 心肌缺血线粒体苯二氯卓类受体动作电位复极至90％的时间有效不应期

DOI：10.3969/j.issn1008-0392.2010.01.005

基金项目:国家"八六三"高技术研究发展计划资助项目，国家"九七三"重点基础研究发展计划资助项目，教育部创新团队计划资助项目，上海市领军人才计划资助项目，国家自然科学基金资助项目

Effects of mBzR inhibition on APD90 and EEP of ischemic cardiomyocytes in rat

LI Fa-jie,LIANG Dan-dan,XIAO Jun-jie,LIU Yi,WANG Shi-long,CHEN Yi-Han

(Key Laboratory of Arrhythmias of Ministry of Education of China, Tongji University, Shanghai 200092, China;;School of Life Science and Technology, Tongji University, Shanghai 200092, China;;Dept. of Cardiology, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China;;Institute of Medical Genetics, Tongji University School of Medicine, Shanghai 200065, China)

Abstract:

Objective To investigate the effects inhibition on 90% of duration of action potential isolated ischemic papillary muscles of rat hearts. of mitochondrial benzodiazepine receptor （mBzR）（APDg0） and effective refractory period （ERP） of Methods The ischemia models were made and then the rats were divided randomly into five groups （n=10）： the normal control group, the ischemia group, 4＇-chlorodiazepam （16 tzM） group, 4＇-chlorodiazepam （32 p~M） group and 4＇-chlorodiazepam （64 IxM） group. The intracellular standard glass microelectrode technique was used, and effects of mBzR inhibition on APD90 and ERP were observed in ischemic papillary muscles of rat hearts. Re- sults Ischemia resulted in progressive shortening of APDgo and ERP. 4＇-chlorodiazepam could blunt APDgo and ERP shortening during ischemia. Conclusion Progressive shortening of APDg0 and ERP in ischemia may be important bases for the genesis of ventricular arrhythmia. 4＇-chlorodiazepam could blunt the shortening of APD~0 and ERP during ischemia, indicating that mBzR inhibition may prevent ischemic ventricular arrhythmias by reducing the reentry.

Key words: ischemic myocardium mitochondrial benzodiazepine receptor 90% duration of action potential effective refractory period