引用本文: |
-
汪进益,范慧敏,刘中民,丁一,$2,$2.大鼠皮肤成纤维细胞MyoD cDNA转染后的形态变化[J].同济大学学报(医学版),2007,(1):6-9. [点击复制]
- .Morphological changes of dermal fibroblasts of rat following transfection with MyoD cDNA in vitro[J].同济大学学报(医学版),2007,(1):6-9. [点击复制]
|
|
摘要: |
目的 探讨运用肌肉转录调节因子(myoblast-de termining,MyoD)基因转染使大鼠皮肤成纤维细胞(dermal fibroblasts,DFs)转化为肌细胞的可能性.方法 采用差速贴壁法培养Sprague-Dawley(SD)大鼠的DFs,利用携带MyoD cDNA的真核表达载体pLenti6/V5- DEST-MyoD转染培养的大鼠DFs,经Blasticidin 筛选2周后挑选转基因单克隆细胞,传代培养,进行细胞形态学观察及细胞免疫化学鉴定.结果 大鼠DFs在导入MyoD cDNA后可见:(1) 部分细胞形态发生改变, 类似骨骼肌细胞,胞体伸展,胞浆颗粒增多,(2) 经抗Desmin抗体的细胞免疫化学检测证实转染后的细胞有下游蛋白Desmin的阳性表达. 结论 DFs能转化为具有潜在功能的类骨骼肌细胞,提示DFs可成为心血管疾病基因治疗的靶细胞. |
关键词: 皮肤成纤维细胞 肌肉转录调节因子 转基因 靶细胞 大鼠 |
DOI: |
修订日期:2006-09-11 |
基金项目:国家自然科学基金资助项目(30471719) |
|
Morphological changes of dermal fibroblasts of rat following transfection with MyoD cDNA in vitro |
|
() |
Abstract: |
Objective To evaluate the possibility of converting cultured rat dermal fibroblasts(DFs) to skeletal muscle cells by transfection with myoblast-determining gene(MyoD cDNA),one of the basic helix-loop-helix myogenic factors.Methods DFs of Sprague-Dawley(SD |
Key words: dermal fibroblasts myoblast-determining transgenes target cell rat |