引用本文: |
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汪进益,范慧敏,刘中民,马亮,$2,$2.犬成年骨骼肌成肌细胞的培养纯化、鉴定及生长特性的研究[J].同济大学学报(医学版),2006,(6):69-72. [点击复制]
- .Culture, Purification, Identification and Proliferative Characteristics of Adult Canine Skeletal Myoblast Cells[J].同济大学学报(医学版),2006,(6):69-72. [点击复制]
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摘要: |
目的建立简便、有效的成年犬骨骼肌成肌细胞(skeletal myoblast cells,SMCS)体外分离培养、纯化和鉴定的方法,并观察犬SMCs的生长特性。方法采用机械分解法与IA型胶原酶和胰蛋白酶的二步酶消化法相结合,分离消化法培养获取犬SMCs,用DMEM培养液培养,以Ficoll梯度液纯化,进行Desmin免疫组化鉴定,并观察细胞形态、生长特性。结果犬SMCs培养后经台盼蓝检查成活率达95%以上,在接种后4~5d增殖达高峰,细胞生长旺盛;Fiooll分离纯化的SMCs纯度较高;Desmin免疫细胞化学染色有97%的SMCs胞浆呈阳性反应。结论改良方法获得的SMCs具有良好的增殖、分化能力;采用FicollU纯化以血清培养,即可获得高产量高纯度的SMCs。 |
关键词: 骨骼肌成肌细胞 细胞培养 纯化 免疫细胞化学 生长特性 |
DOI: |
修订日期:2006-07-10 |
基金项目:上海市科技发展基金资助项目(02ZB14078) |
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Culture, Purification, Identification and Proliferative Characteristics of Adult Canine Skeletal Myoblast Cells |
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Abstract: |
Objective Establish a thrifty, convenient and practical procedure of the culture, purification, identification and observe the prolif erative characteristics of adult canine skeletal myoblasts cells ( SMCs) for cellular cardiomyoplasty in vitro. Methods A |
Key words: adult canine skeletal myoblast cells cell culture purification immunocytochemistry proliferative characteristics. |