| 引用本文： |
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赵敏,$2,于永春,段建敏,王红玲.死亡相关蛋白激酶启动子区甲基化状态与膀胱癌的关系[J].同济大学学报（医学版）,2006,(3):29-32. [点击复制]
- .Association of CpG Island Methylation Status of Deathassociated Protein Kinase with Bladder Cancer[J].同济大学学报（医学版）,2006,(3):29-32. [点击复制]
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| 摘要: |
| 目的探讨死亡相关蛋白激酶(death-associated protein kinase,DAPK)在膀胱癌中CpG岛的甲基化状态及其意义。方法收集40对新鲜的膀胱癌组织及其癌周正常组织;采用甲基化特异性PCR(methylation specific PCR,MSP)检测DAPK基因启动子区CpG岛的甲基化状态;并对MSP产物进行TA克隆。结果40对癌组织及其癌周正常组织中,7例癌组织检测到DAPK异常甲基化,1例癌周正常组织检测到异常甲基化(7/40vs.1/40,P>0.05);原发性与复发性膀胱癌中的甲基化阳性率分别为2/15、5/25,二者差异无显著性(P>0.05);在不同分级、分期膀胱癌中的差异均无显著性(P>0.05)。结论DAPK启动子在膀胱癌中的甲基化阳性率较低,并且与膀胱癌的分级、分期无相关性,因而在选择分子学指标辅助膀胱癌早期诊断时,DAPK的甲基化检测需慎用。 |
| 关键词: 膀胱癌,死亡相关蛋白激酶,甲基化特异性PCR |
| DOI： |
| 修订日期：2005-11-18 |
| 基金项目: |
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| Association of CpG Island Methylation Status of Deathassociated Protein Kinase with Bladder Cancer |
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| Abstract: |
| Objective To investigate CpG island methylation status of death-associated kinase protein(DAPK) in bladder cancer and its meaning.Methods Forty fresh bladder tumor tissues and paired adjacent normal tissues were obtained from cystectomy specimens.The CpG |
| Key words: bladder cancer,death-associated protein kinase,methylation specific PCR |
