| 摘要: |
| 目的探讨骨肉瘤肿瘤细胞自身血管形成基因表达,为靶向骨肉瘤肿瘤血管治疗提供研究基础。方法利用RT-PCR技术对骨肉瘤肿瘤细胞(MG63、U2OS)血管形成相关基因进行检测,并同内皮细胞(ECV304)相比较。结果骨肉瘤肿瘤细胞和内皮细胞相类似,自身在mRNA水平均能表达VEGF和其受体KDR,受体Tie-2,以及EPHA2,Thrombin,VE-Cadherin等血管形成相关基因。结论骨肉瘤肿瘤细胞表达成血管细胞表型,具有类胚胎表型的逆转,这可能是骨肉瘤富含血供基础,在肿瘤的靶向治疗中,具有进一步的研究前景。 |
| 关键词: RT-PCR,骨肉瘤细胞,基因,血管形成 |
| DOI： |
| 修订日期：2005-12-01 |
| 基金项目:国家自然科学基金资助项目(30271314) |
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| The Gene Expression of Osteosacoma Cells Associated with Tumor Vasculargenesis |
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| () |
| Abstract: |
| Objective To explore the gene expression of osteosacoma cell associated with tumor vascular genesis,the study basis was provided for the therapeutic targets at the vascular channels of osteosacoma.Methods Gene expression associated with vascular channel i |
| Key words: RT-PCR,osteosarcoma cell,gene,vascular genesis |
