| 引用本文： |
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唐翠兰,彭国平,陈智,朱海红,郑敏.慢性乙型肝炎患者炎症相关差异表达基因cDNA文库的构建和筛选[J].同济大学学报（医学版）,2006,(1):4-8. [点击复制]
- .Construction and Analysis of cDNA Library Accociated with Inflammation of Chronic Hepatitis B[J].同济大学学报（医学版）,2006,(1):4-8. [点击复制]
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| 摘要: |
| 目的筛选慢性乙型肝炎(chron ic hepatitis B,CHB)患者与无症状乙型肝炎病毒携带者(asymptom atic HBVcarriers,ASCs)表达差异的基因。方法分离CHB及ASCs的外周血单个核细胞(peripheral b lood mononuc lear cells,PBMC)并提取RNA,SMART技术合成cDNA,采用抑制消减杂交(suppression subtractive hybrid ization,SSH)技术构建差异表达基因cDNA文库,进一步采用dot b lot筛选,得到差异表达的基因。结果经过筛选共获得了22个阳性克隆,通过序列分析,发现它们代表22个基因,在CHB表达增高的基因多是和炎症相关,在ASCs表达增高的基因中,多为新基因。结论成功构建了CHB与ASCs的消减文库并初步分析这些差异表达基因的意义,对于阐明CHB的发病机制及其治疗都有重要意义。 |
| 关键词: 慢性乙型肝炎,无症状乙型肝炎病毒携带者,抑制消减杂交 |
| DOI： |
| 修订日期：2005-09-04 |
| 基金项目:浙江省科技厅重点资助项目(2003C13015) |
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| Construction and Analysis of cDNA Library Accociated with Inflammation of Chronic Hepatitis B |
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| Abstract: |
| Objective To identify differentially expressed genes between chronic hepatitis B(CHB) patients and asymptomatic HBV carriers(ASCs).Methods cDNA was synthesized by SMART technology from RNA of peripheral blood mononuclear cells(PBMC),subtracted libraries w |
| Key words: chronic hepatitis B,asymptomatic HBV carriage,suppression subtracted hybridization |
