| 引用本文： |
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张文萍,谈月娣,张天祥,蒋丽珍.转FasL基因卵巢癌HO-8910细胞系的建立[J].同济大学学报（医学版）,2005,(3):22-. [点击复制]
- .Construction of HO-8910-FasL, a Cell Line with Gene Transfer of Fas L of Ovarian Cancer[J].同济大学学报（医学版）,2005,(3):22-. [点击复制]
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| 摘要: |
| 目的建立表达转FasL基因卵巢癌细胞系，为以FasL为靶点治疗卵巢癌提供理想细胞模型。方法应用分子克隆技术，将人FasL cDNA片段正向插入逆转录病毒载体pLXSN构建重组质粒pL(hFasL)SN，转染包装细胞PA317收获假病毒上清，磷酸钙法转染卵巢癌HO-8910细胞筛选建系，酶切PCR鉴定、流式细胞仪(FCM)法检测。结果酶切鉴定证实重组质粒pL(hFasL)SN中存在FasL，PCR方法从转染细胞HO-8910-FasL基因组中扩增出0．86kb片段，FCM检测显示FasL表达增加40．32％，HL-60细胞与HO-8910-FasL细胞共培养HL-60细胞凋亡增加25．65％。结论应用逆转录病毒法建立了稳定高表达FasL并具功能的HO-8910-FasL细胞系。 |
| 关键词: 卵巢肿瘤 转基因 FasL HO-8910细胞系 |
| DOI： |
| 修订日期：2004-12-20 |
| 基金项目: |
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| Construction of HO-8910-FasL, a Cell Line with Gene Transfer of Fas L of Ovarian Cancer |
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| Abstract: |
| Objective To construct a HO-8910 cell line with gene transfer of FasL of ovarian cancer and to give a good model for studying ovarian cancer.Methods Human FasL cDNA was inserted into reombinant retrovirus vector plasmidpLXSN by subcolone method.By using c |
| Key words: ovarian neoplasms,gene transfer,FasL,HO-8910 cell line |
