摘要: |
目的探索结膜上皮细胞体外培养的最佳方法,研究其生长特性,为毒理实验及体外结膜上皮移植提供基础。方法分别用3种培养方法:(1)组织块培养法;(2)混合肖化法;(3)组织块消化后贴壁法来培养人、兔的结膜上皮细胞,观察细胞状态、不同部位的长生特性等,并尝试在羊膜、饲细胞层上培养建系。结果3种培养方法中,组织块培养法能获得较纯的结膜上皮细胞但生长缓慢,消化法能获得大量细胞,生长较快,但常常混有其它杂细胞,组织块消化后贴壁法获得细胞量较少。观察到来自兔、睑结膜及穹隆部结膜上皮的细胞生长速度较快,细胞量多,来自球结膜的细胞生长较慢。应用间接免疫荧光染色,人结膜上皮细胞K13染色阳性。结论组织块培养法及混合消化法皆能培养结膜上皮细胞,各有利弊。 |
关键词: 结膜上皮 细胞培养 免疫组化 |
DOI: |
修订日期:2004-10-15 |
基金项目: |
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Primary Culture of Conjunctival Epithelial Cells of Human and Rabbit in Vitro |
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Abstract: |
Objective To study the best culture method an d growth characteristics of conjunctival epithelial cells of human and rabbits in vitro and to provide a model as a base for toxicity experiment and clinical tran splantation.Methods 3 culture methods were |
Key words: conjunctival epithelium,cell culture,immuno histochemistry |