| 引用本文： |
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侯文辉,丁祖泉,邢军,何俊民.竞争PCR用于乙肝病毒DNA定量检测初探[J].同济大学学报（医学版）,2004,(6):509-512. [点击复制]
- .Detection of Serum Hepatitis B Virus DNA by Quantitative Competitive Polymerase Chain Reaction[J].同济大学学报（医学版）,2004,(6):509-512. [点击复制]
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| 摘要: |
| 目的 评估竞争PCR这种检测方法的临床有效性。方法 用竞争PCR和荧光定量PCR两种方法对同一组患者血清HBVDNA进行了定量检测。竞争PCR是通过聚合酶链反应过程中竞争子和目的乙肝病毒DNA竞争同一反应管内的同一引物 ,通过凝胶成像比较目的乙肝病毒DNA和已知量竞争子的亮度 ,推测出目的DNA的含量。再将其结果与荧光定量PCR的结果相比较。结果 竞争PCR与荧光定量PCR检测的结果一致。结论 运用竞争PCR的方法对乙肝病毒DNA进行定量检测结果精确、成本较低 ,简便快速、易于操作 ,可推广使用。 |
| 关键词: 乙肝病毒DNA,竞争子,竞争PCR |
| DOI： |
| 修订日期：2004-06-28 |
| 基金项目: |
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| Detection of Serum Hepatitis B Virus DNA by Quantitative Competitive Polymerase Chain Reaction |
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| Abstract: |
| Objective To evaluate the clinical usefulness of competitive PCR.Methods Competitive PCR and fluorescence-quantitative PCR were compared through quantitative detection in the same patients' serum HBV DNA.Competitive PCR means target HBV DNA can rivalize |
| Key words: hepatitis B virus,competitor,competitive PCR |
