| 引用本文： |
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蔡海东,娄继权,邱桂林,黄飚,顾伟中.99mTc标记结肠癌单克隆抗体片段CEA．Fab的研制方法[J].同济大学学报（医学版）,2004,(4):343-344348. [点击复制]
- .Labeling of Monoclonal Antibody CEA-Fab Segment Against Colon Cancer with 99mTc[J].同济大学学报（医学版）,2004,(4):343-344348. [点击复制]
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| 摘要: |
| 目的 探讨一种高效便捷的99mTc CEA Fab标记化合物的研制方法。方法 采用 2 亚氨噻酚盐酸盐修饰CEA Fab ,通过GH转换 ,将99mTc标记在结肠癌单克隆抗体CEA Fab上。结果 已修饰抗体的标记率为 93.5 %,99mTc胶体含量为 2 .5 %,标记化合物有良好的稳定性和免疫活性。结论 成功地建立了结肠癌单抗片断CEA Fab的99mTc标记方法 ,该技术可以应用于其他抗体片段标记领域。 |
| 关键词: 癌胚抗原,单克隆抗体,~(99m)Tc,标记 |
| DOI： |
| 修订日期：2003-11-05 |
| 基金项目: |
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| Labeling of Monoclonal Antibody CEA-Fab Segment Against Colon Cancer with 99mTc |
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| Abstract: |
| Objective To provide an accurate and convenient labeling method of 99m Tc CEA Fab for radionuclide imaging of colon cancer.Methods The monoclonal antibody CEA Fab segment was modified by iminothiolane and the ligand was exchanged from |
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