| 摘要: |
| 目的研究多种酶在不同器官内吞体膜上的活性。方法用细胞化学方法,以Ce3 或Pb2 作捕捉剂,分别检测碳酸酐酶,Mg2 -ATP酶,Na -K -ATP酶,5’-核苷酸酶,NADPH oxidase在大鼠肾近曲小管上皮细胞、肝细胞以及人体嗜中性白细胞内吞体膜上的酶活性。结果上述碳酸酐醇,Mg2 -ATP酶,Na -K -ATP酶,5’-核苷酸酶及NADPH oxidase分别在大鼠肾脏近曲小管,肝脏及人体白细胞的内吞体膜上都有酶活性存在。结论 内吞体功能复杂,它可能与细胞的pH值,水盐代谢,能量、蛋白质的代谢以及活性氧的产生等都有一定的关系。 |
| 关键词: 内吞体,细胞化学,酶,大鼠 |
| DOI： |
| 修订日期：2003-08-06 |
| 基金项目: |
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| The Localization of Several Enzyme Activities on the Membrane of Endosome |
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| Abstract: |
| Objective To investigate cytochemical localization of enzymes on the membrane of endosome in different organs. Methods To use Ce3 or Pb2 as capture agents to detect carbonic anhydrase, Na - K - ATPase, Mg2 -ATPase, 5'-nucleotidase and NADPH oxidase a |
| Key words: endosome,cytochemistry,enzyme,rats |
