摘要: |
目的:探讨MTT法检测肿瘤细胞化疗敏感性的影响因素。方法:选用肝癌细胞株SMMC7721和BEL7402,采用正交实验设计,分析MTT法检测肿瘤细胞化疗敏感性的条件因素对药敏检测结果的影响。结果:肝癌细胞数与生成的甲Zuan(formasan)的光密度(OD)值呈线性正相关。肝癌细胞数、药物浓度,药物作用时间是影响MTT法检测肿瘤抑制率的重要因素。正交设计应用于MTT法检测肿瘤细胞化疗敏感性,与平行设计的检测结果相关一致。结论;MTT法检测肿瘤细胞化疗敏感性,具有简易、快速、重复性较好等优点,但检测条件(肿瘤细胞数,药物浓度,药物作用时间)可能会严重影响肿瘤抑制率检测结果的和药敏判断。 |
关键词: MTT法 检测 肝癌 化疗敏感性 药物疗法 |
DOI: |
修订日期:2001-09-30 |
基金项目: |
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Study of MTT Colorimetric Assay as Chemosensitivity Test for Hepatic Cancer Cell |
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Abstract: |
Objective To investigate the effects of conditions on chemosensitivity test using MTT colorimetric assay.Methods The effects of conditions on chemosensitivity test for hepatoma cells with MTT colorimetric assay were surveyed. Hepatoma cell line SMMC7721 |
Key words: liver tumor cells,chemosensitivity,MTT colorimetric assay,orthogonal experiment design |