| 摘要: |
| Objective To
explore the possible mechanism of vitamin E against atherosclerosis through its preventive
effect on tumor necrosis factor (TNF) inducing endothelial cell injury.Methods Bovine
aortic endothelial cells were incubated in culture media with TNF-alpha (400 U/ml) and
vitamin E (12.5 microM, 25.0 microM and 50.0 microM respectively) for 72 h.The morphology,
proliferation, apoptosis and adherence of endothelial cells were investigated with
phase-contrast microscope, MTT method, flow cytometry analysis and cell count
respectively.Results Compared with TNF group, the phenomena of
endothelial cell deformity and shedding were slighter, the number of adhered HL60 monocyte
cells and G1-phase cells were less, and the inhibitory rate and the apoptotic
peaks were significantly lower in vitamin E groups.Conclusion The
results suggested that vitamin E has definite protective effects on injury induced by TNF
as shown in morphology, proliferation and apoptosis of endothelial cells in vitro. |
| 关键词: 维生素E 内皮细胞 肿瘤坏死因子 细胞增殖 细胞凋亡 |
| DOI： |
| 修订日期：1998-11-04 |
| 基金项目: |
|
| Rolesof Vitamin E on the Protection against Aortic EndothelialCell Injury Induced by Tumor Necrosis Factor in vitro |
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| Abstract: |
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| Key words: |
