| 引用本文： |
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陈浩宏,游根娣,顾芝萍,曹霖.多种化合物对体外培养人滋养层细胞
和蜕膜细胞的损伤作用[J].同济大学学报（医学版）,1999,(1):10-. [点击复制]
- .Observation on the Detrimental Effects of 28 Compoundsto the Human Trophoblast Cells and Decidual Cells In Vitro[J].同济大学学报（医学版）,1999,(1):10-. [点击复制]
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| 摘要: |
| Objective To
observe the detrimental effects of 6 known antifertility compounds and 22 natural
compounds which may have the antifertility activity to the human trophoblast cells and
decidual cells, to check the model for screening new antifertility drugs, and to screen
new active antifertility compounds.Methods The culture method for human
trophoblast cells and decidual cells and hCG radioimmunoassay were applied to observe and
screen antifertility activity of microcompounds.Results Antifertility
compound Ru486 (100 μg/ml), Tamoxifen (20 μg/ml), Anordrin (100 μg/ml)
and Trichothanthin (50 μg/ml) not only injured the morphology of human trophoblast
cells, but also inhibited the hCG secretion of them, whereas Pseudolaric acid B (100 μg/ml)
and Yuanhuacine A ( 0.5 μg/ml) only altered the morphology of trophoblast cells.Among
22 natural compounds detected, 100 μg/ml of Hml, Hmlo, TR4, T2
were not only detrimental to the trophoblast cells but also inhibitory to the hCG
secretion.Antifertility compounds Ru486(10 μg/ml), Anordrin (60 μg/ml),
Pseudolaric acid B (50 μg/ml) and Yuanhuacine Ⅰ (0.5 μg/ml) were detrimental to
human decidual cells, whereas Trichothanthin did not alter the morphology of decidual
cells.Among 22 natural compounds detected, TR4 was evidently detrimental to
decidual cells, whereas Zh-1 and other 19 compounds had no effect.Conclusion Human
trophoblast cells and decidual cells in vitro may be taken as the model for screening
antifertility drugs; TR4 was evidently detrimental to the trophpblast cells and
decidual cells and deserves further research. |
| 关键词: 滋养层细胞 蜕膜 药物筛选抗早孕化合物 细胞培养 米非司酮 |
| DOI： |
| 修订日期：1998-10-13 |
| 基金项目: |
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| Observation on the Detrimental Effects of 28 Compoundsto the Human Trophoblast Cells and Decidual Cells In Vitro |
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